SELECTION OF REFERENCE GENES FOR TRANSCRIPTION ANALYSIS
FOR MYOCARDITIS STUDIES
A.L. Runov,1,* E.V. Kurchakova,1 D.A. Khaschevskaya,2
O.M. Moiseeva,2 M.S. Vonsky 1
1 Institute of Cytology RAS and 2 Federal V.A. Almazov Medical Research Centre, St. Petersburg;
* e-mail: tsinakan@gmail.com
Myocarditis is defined as myocardial inflammation, followed by cardiomyocyte necrosis. Diagnostics of
myocarditis is based on unsafe and complicated method of endomyocardial biopsy (EMB). Development of
myocarditis might alter gene expression not only in cardiac but in peripheral blood cells (PBC) as well. So,
transcription profiles can be considered as possible biomarkers for the given pathology. At the moment, there
are no reference genes defined for expression analysis in myocarditis studies. In this study, we analyzed mRNA
content of six housekeeping genes in EMB and PBC samples. An algorithm for processing qPCR results obtained
under the limited amount of sample is proposed. Set of GAPDH and HPRT1 genes has been selected for
normalization of gene expression profiles in cardiac tissue and blood cells under the studies of myocarditis.
Key words:
myocarditis, mRNA, RT-qPCR, reference gene, gene expression
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