MESENCHYMAL STEM CELLS INFLUENCE ON LEUCOCYTES ALLERGEN-SPECIFIC
REACTIONS IN CASE OF ATOPIC HYPERSENCITIVITY
A.A. Aisenstadt,1,2,* O.V. Supilnikova,1,2 V.V. Bagaeva,1 A.B. Smoljaninov,1,2
M.P. Samoylovich,3 V.B. Klimovich 3
1 Stem Cell Bank Pokrovsky, Ltd.,
2 Northwestern I.I. Mechnikov State Medical University and
3 Russian Research Centre for Radiology and Surgical Technologies, St. Petersburg;
* e-mail: aizendt@gmail.com
Buffy coat samples containing lymphocytes, monocytes and granulocytes, were obtained from the peripheral
blood of 16 donors who had clinical manifestations of atopic hypersensitivity in their medical background.
After ex vivo incubation with donor-specific allergens, the percentage of B- and T-lymphocytes and natural killers
(NK) remained unchanged. Buffy coat incubation with allergens induced production of IgE and IL-4 in all
studied samples. In 13 out of 16 cases the reaction to contact with an allergen was also evident in the increasing
of T-activated lymphocytes (CD3+, HLA-DR+) subpopulation. Co-cultivation with MSC from bone marrow,
adipose tissue and umbilical cord resulted in blocking of allergen-induced IgE and IL4 secretion and HLA-DR+
T-lymphocytes subpopulation increase. There were no significant differences in the effect of MSCs, isolated
from three different sources, on allergen-specific responses of leukocytes. Co-culturing of leukocytes with
MSCs from all three sources led to an increase in the content of regulatory T-lymphocytes by an average of
30 %. Thus, the immunomodulatory activity of MSCs in vitro results in blocking of the effector part of allergic
reactions.
Key words:
mesenchymal stem cells, hypersensitivity reaction, co-cultivation
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