A DEVICE FOR THE VACUUM TREATMENT OF HISTOLOGICAL MATERIAL
A.Yu. Budantsev
Institute of Theoretical and Experimental Biophysics RAS, Puschino, 142290;
e-mail: budantsev@mail.ru
A device to carry out all types of vacuum infiltration in a wide temperature range. The device is easy to use and allows you to simultaneously conduct the infiltration of a large number
of tissue samples. The main element of the device is a temperature-controlled cuvette, the temperature of which is set in the range of 23-65 °C. The design of the cell allows pumping
air from the cell to the 50-100 mm Hg. In practice, histolo-gical methods are used to remove the vacuum infiltration of air from the tissue fixation (plant tissue), to accelerate the
penetration of fasteners into the tissue. To carry out such a procedure, syringes, water pump and other hand-held devicesare used. Only vacuum infiltration allows a fill lyophilized
tissue in paraffin. Efficiency of the described device is checked during the experiments at various fixing plant tissue and nervous tissue filling lyophilized in paraffin.
Key words:
fixation of histological materials, infiltration of histological materials, vacuum embedding tissue in paraffin
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