FEATURES OF RAT CELLS KARYOTYPIC ABNORMALITIES IN RAT CELLS IN THEIR TRANSFORMATION IN VITRO
N.̀. Yartseva,1,* R.F. Fedortseva 2
1 Institute of Cytology RAS and 2 FSBI «The A. M. Nikiforov Russian Center for Emergency
and Radiation Medicine» EMERCOM of Russia, St. Petersburg;
*e-mail: Yartseva@mail.cytspb.rssi.ru
Neoplastic transformation of cells is characterized by karyotypic abnormalities involving aneuploidy, quantitative
changes, as well as multiple clonal rearrangements of the number and structure of chromosomes. It is
probable that chromosomes are one of the mechanisms of cells immortalization and transformation. Despite
many years of study of chromosomal rearrangements, data on primary chromosomal rearrangements in early
stages of transformation is still insufficient. We examined karyotypic abnormalities in embryonic rat fibroblasts
in both the spontaneous transformation and transformation by oncogenes at different passages in vitro. Literature
data and results of our cytogenetical analysis of rat cells lines established by different methods of transformation
of cells of different tissue origin in vitro have shown that cell karyotype at early passages may either be
normal or acquire diverse clonal chromosomal abnormalities. At later passages, other chromosomes of karyotype
are involved in new rearrangements. Despite this, some of the lines do not acquire the malignant phenotype
and remain to be immortalized. The role of instable chromosomes and their loci in immortalization and transformation
of cells is discussed.
Key words: chromosomal rearrangements, karyotype, cell lines, transformation
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