APOPTOSIS AND NECROSIS OF LYMPHOCYTES INDUCED BY UV-RADIATION
IN THE PRESENCE OF AUTOLOGICAL PLASMA
V.G. Artyukhov,1 O.V. Zemchenkova,2 O.V. Basharina,1 S.V. Ryazantsev,2 M.V. Pashkov 2
1 Department of Biophysics and Biotechnology Voronezh State University and
2 N.N. Burdenko Medical Academy, Voronezh;
2e-mail: zov-bio@mail.ru
The influence of UV-light (240—390 nm) in doses 151—3020 J/m2 on the nature of the death of lymphocytes
cells of donor’s blood (using markers of apoptotic and necrotic death of cells) and on the level of expression
of the marker of apoptotic pre-preparation — CD95-receptor has been investigated. We have shown that
UV-radiation increases expression of CD95-receptors which is caused mainly by de novo synthesis of the receptors.
It has been revealed that during daily incubation of photo-modified lymphocytes (151 and 755 J/m2)
without autological blood cell death occurs by receptor-involved apoptosis. Exposure to high doses of radiation
(1510 and 3020 J/m2) causes massive necrotic death of immunocytes. The use of autologous blood plasma during
incubation of photo-modified lymphocytes allows decreasing the number of both apoptotic and necrotic
cells.
Key words: apoptosis, CD95, lymphocytes, necrosis, UV-radiation
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