ZAJDELA HEPATOMA CELLS CULTURED IN VITRO
N.P. Teryukova,* G.I. Blinova, V.A. Ivanov
Institute of Cytology RAS, St. Retersburg;
* e-mail: npter@yandex.ru
Monolayer (ZH-ad) and suspension (ZH-fl) cell cultures obtained in results of explantation of ascitic Zajdela hepatoma in culture in vitro are differing in levels of cell
differentiation and tumorigenicity. Using tumor-specific rabbit immune serum tumor-associated antigens were detected in the fraction of plasma membrane of ZH-fl cells,
synthesis of that are reduced or suppressed in ZH-ad cells. Intraperitoneal injection of ZH-fl cells as few as 0,5·106 cells/rat are enough for development of
ascite tumor, while minimal dose for ZH-ad cells increase to 20·106 cells. By means of clonogenic analysis of ZH-fl cells three types of generated clones were
revealed - nonadhesive sphere colonies and two types of monolayer clones, that are diversity on proliferative potential, shape of colonies and cell composition. When the critical
size of spheres was reached there is its disintegration with dissociation of single cells or islands of different proportions, part of which attached to the plate with monolayer
formation. Three clonal cell lines were obtained: 1C - in result of expansion of sphere clone cells, 4G and 10E - from monolayer clones. Our results demonstrate a clonogenic
origin of multicellular islands of ascitic hepatoma Zajdela.
Key words: hepatoma, cloning, tumor-associated antigens, cancer stem cells, tumorigenicity
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