INFLUENCE OF MESENCHYMAL STROMAL CELLS ON B-CELL LINE GROWTH AND IMMUNOGLOBULIN SYNTHESIS
M.P. Samoylovich,1 A.A. Pinevich, O.A.Shashkova, N.L. Vartanian.,
L.N. Kiseleva, V.B. Klimovich
Russian Research Center for radiology and surgical technologies, St-Petersburg;
1 e-mail: mpsamoylovich@gmail.com
A number of publications contain contradictory data about the influence of mesenchymal stromal cells (MSC) on B-lymphocyte growth, differentiation and production of
immunoglobulins (Ig). The aim of the study was the investigation of influence of MSC derived from adipose tissue of healthy donors and cancer patients on the proliferation
and Ig synthesis of lymphoblastoid cell line Namalva and myeloma cell line U266. Cocultivation of Namalva cells with MSC stimulated their proliferation, decreased the doubling
time and the minimal effective seeding dose and therefore made cloning of these lymphoblastoid cells possible. The presence of MSC supported survival and proliferation of
Namalva cells cultivated in growth factor deficient medium. MSC also stimulated proliferation of U266 myeloma cells. Both MSC derived from adipose tissue of healthy donors
and patients with breast cancer effectively stimulated B-cell lines proliferation. Presence of MSC in mixed cultures had no influence on the production of IgM or IgE by Namalva
or U266 cells respectively. Cocultivation of Namalva or U266 with MSC resulted in formation of close intercellular contacts between cells of both types.
Key words: mesenchymal stromal cells, B-cell lines, cocultivation, Ig synthesis
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