MESENCHYMAL STEM CELLS OF HUMAN ENDOMETRIUM DO NOT UNDERGO SPONTANEOUS TRANSFORMATION DURING LONG-TERM CULTIVATION
A.P. Domnina, I.I. Fridlianskaia, V.I. Zemelko, N.A. Pugovkina, Z. V. Kovaleva,
V.V. Zenin, T.M. Grinchuk, N.N. Nikolsky
Institute of Cytology RAS, Saint-Petersburg;
e-mail: aldomnina@mail.ru
Mesenchymal stem cells isolated from human endometrium (eMSC) are perspective source of stem cells for regenerative medicine. Large amount of these cells
accumulated by in vitro cultivation is usually required for transplantation into patients. We established several cell eMSC lines and cultivated them during long period of time t
o examine the possibility of their spontaneous transformation. All cell lines demonstrate limited lifespan, undergo replicative senescence and die. Karyotypic analysis on
different passages reveals that most cells display karyotypic stability. Thus, extended in vitro cultivation of eMSCs does not lead to spontaneous transformation that makes
therapeutic application of these cells safety for patients. During long-term cultivation eMSCs sustain the expression of surface markers.
Key words: human mesenchymal stem cells, endometrial stem cells, spontaneous transformation, karyotype of eMSCs
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