OPTIONAL PROTOCOLS OF HEMATOPOIETIC STEM CELL EXPANSION IN VITRO
R.I. Dmitrieva,1 S.V. Anisimov 1,2
1 V.A. Almazov Federal Center for Heart, Blood & Endocrinology, Saint-Petersburg;
2 Institute of Cytology, Russian Academy of Sciences, Saint-Petersburg;
e-mail: renata.i.dmitrieva@gmail.com
Hematopoietic stem cells (HSC) effectively and continuously renew a full spectrum of blood cell populations. Bone marrow and umbilical cord blood stem cells
transplantation (SCT) restore hemopoiesis, when used in various hematological and oncohematological disorders in adults and children. However, wider clinical application
of effective SCT-based approaches is limited by the low number of the primitive HSC in the available biospecimens. A development of effective protocols of HSC expansion
in vitro is therefore necessary. In this review, a concept of bone marrow hematopoiesis niche being a complex cellular system is discussed; a comparative analysis
of various methods for HSC expansion in vitro is provided. The review is illustrated by the author's own data supporting application of various feeder cell types for human
HSC expansion in vitro.
Key words: hematopoietic stem cells, mesenchymal stem cells
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