EXAMINATION CYTOTOXIC EFFECT OF ANTI-CANCER DRUG DOXORUBICIN ON HUMAN EMBRYONIC STEM CELLS
Kozhukharova I.V., Grinchuk T.M., Pugovkina N.A., Kovaleva Z.V.,
Alekseenko L.L., Nikolskiy N.N.
Institute of Cytology RAS, St.Petersburg;
e-mail: kojuxarova@mail.ru
Cytotoxic effect of anti-cancer drug, doxorubicin (DR), has been examined on human embryonic stem cells (ESC) C910 and fibroblasts spontaneously differentiated from these cells.
The fibroblasts retained diploid karyotype. It was found that ESC are more sensitive to DR than fibroblasts: DR dose killing 20 % cells was 0.01 and 0.1 mg/ml, respectively. DR induced
ESC apoptotic death and reduced both ESC and fibroblast proliferation. Unlike fibroblasts DR reversibly inhibited ESC proliferation. Thus, we have demonstrated that ESC and their
differentiated derivates differ their sensitivity and response to the genotoxic agent.
Key words: human embryonic stem cells, doxorubicin, cytotoxicity
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