INFLUENCE OF EXOGENOUS EMBRIONIC FIBROBLASTS ON COLLAGEN TYPE I AND TYPE III RATIO IN THE TISSUES OF PARAPROSTHETIC
CAPSULE (EXPERIMENTAL WORK)
Ivanov I.S., Lazarenko V.A., Ivanov S.V., Goryainova G.N., Ivanov A.V.
Kursk State Medical University;
e-mail: ivanov.is@mail.ru
Nowadays, the use of synthetic prosthesis is obligatory method in surgical treatment of large and giant ventral hernias. We have fulfilled comparative investigation of the dynamics
of collagen Type I to Type III ratio in paraprosthetic region in mice when using implants, Esphyl - polyethylene, Ecophlon - polytetrafluorethylene, and Uniflex-polyvinylidenfluoride,
with a one- and two-time introduction of cultured fibroblasts into paraprosthetic region at different times after implantations and without the introduction. Use of Sirius Red dye staining
and polarization microscopy revealed that, in short period (10 days after endoprosthesis implantation), the implication of fibroblasts and the number of implications did not affect the
collagen Types ratio. In later stages (30-60 days after endoprosthesis implantation) a significant increase in collagen Type I was observed when using all the materials. The ratio of
collagen Type I to Type III was maximal at all stages of the experiment in the case of the implant Uniflex. Double implication of exogenous fibroblasts accelerated the increase in the
collagen Type I to Type III ratio to a greater extent.
Key words: post operation hernia, collagen Types I and III, polarization microscopy, net endoprosthesis, fibroblasts
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