STIMULATORY EFFECT OF THE CORD BLOOD FRACTION BELOW 5 kDa AND "ACTOVEGIN" ON THE GROWTH OF CONTINUOUS CELL LINES
A. K. Gulevsky,1 A. V. Trifonova,1 A. A. Lavrik 2
1 Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine, Kharkov, and
2 State Research Control Institute of Biotechnology and Microorganisms Strains, Kiev, Ukraine;
1 e-mail: inessagvazava@hotmail.ru
The influence of the cattle cord blood fraction with molecular weight of components below 5 kDa on adhesive and proliferative properties of continuous cell lines BHK-21
clone q3/04 and PK-15-IECVM was investigated in comparison with the preparation "Actovegin". It was shown that adding this fraction as well as "Actove-gin" to growth media
did not affect the efficiency of culture attachment, promoted culture spreading, improved cell morphology and stimulated mitotic activity of the cultures. The efficiency of the cord
blood fraction below 5 kDa estimated by the induces studied was found to be higher than the "Actovegin" efficiency when cells being cultivated in the media different contents of
blood serum.
Key words: cord blood fraction below 5 kDa, "Actovegin", continuous cell lines, adhesion, mitotic regimen, proliferation
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