CULTIVATION OF CELLS ON A SURFACE COVERED BY MICROSPHERES WITH COUPLED HISTONES
O. A. Goryukhina,1,* S. V. Martyushin,2 M. I. Blinova,3 G. G. Poljanskaya,3
O. A. Cherepanova,3 G. P. Pinaev 3
1 Department of Biochemistry of St. Pctcrsbutg State University,
2 State Research Institute of Highly Pure Bioprcparations
and 3 Institute of Cytology RAS, St. Petersburg;
*e-mail: pushkeen@gmail.com
The use of histones for modification of the surface intended for cultivation of cells was studied. The work was carried out on the cell line 293 of human embryonic kidney
transformed by adenovirus (Ad5) and on the cell line BALB/3T3 clone A31 of mouse spontaneous transformed embryonic fibroblasts. We analyzed interaction of cells with
histones of different types put on a hydrophobic surface or on dextran microspheres with diameters of 1.0 μ. It was shown, that all histones studied possessed
adhesive ability, but their complexes consisting of total and core histones rendered the best influence on adhesion, morphology and growth of the cells in culture. Thus,
cross-linked conjugates of histones immobilized on microspheres promoted in a greater degree formation of a network of cellular structures due to formation of intracellular
contacts and simultaneous interaction of cells with several microspheres. Comparing with BALB/3T3 clone A31 the cell line 293 showed significant increase in proliferative
activity in 11 days of cultivation on microspheres covered with cross-linked conjugates of histones. Our investigations have shown that the microspheres covered with
cross-linked conjugates of histones can be used in the further at creation of the three-dimensional porous matrices intended for in vitro formation of tissue-like cellular
structures in them.
Key words: histones, microspheres, adhesion, spreading, proliferation of cultivated cells
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