TRPV5 AND TRPV6 CALCIUM CHANNELS IN HUMAN T CELLS
I. O. Vassilieva, Yu. A. Negulyaev, I. I. Marakhova, S. B. Semenova 1
Institute of Cytology RAS, St. Petersburg;
1 e-mail: Adanina@rambler.ru
The recent cloning of the special calcium channels TRPV5 and TRPV6 (transient receptor potential vanilloid channels) provided the molecular base for the
studying of new candidate of calcium influx in non-excitable cells. Using RT-PCR technique we obtained endogenous expression of the mRNAs trpv5 and trpv6 in
lymphoblast leukemia Jurkat cells and in human blood primary T lymphocytes. Additionally, Western blot analysis showed TRPV5 proteins in both the whole lysate
and in the crude membrane preparations from Jurkat cells and normal T lymphocytes. The using of the immunoprecipitation revealed TRPV6 proteins in Jurkat cells,
whereas in normal T lymphocytes TRPV6 was not detected. The expression pattern and the selective бр2+ permeation properties of TRPV5 and
TRPV6 channels indicate an important role of these channels in the бр2+ homeostasis and probably in malignant transformation of blood cells.
Key words: TRPV5, TRPV6 calcium channels, RT-PCR, expression of channel proteins, human T cells
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