CHANGES IN THE DNA CONTENT IN EGF-STIMULATED CILIATE TETRAHYMENA PYRIFORMIS UNDER EFFECT OF CAFFEINE, KCL, AND INHIBITORS OF PLC AND PKC
I. V. Shemarova,1 G. V. Selivanova,2 T. D. Vlasova 2
1 I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry RAS and 2 Institute of Cytology RAS, St. Petersburg;
e-mail: irina@lis.mail.iephb.ru
It has been established that DNA content in ciliata Tetrahymena pyriformis changes under effect of the caffeine, and PLC and PKC inhibitors (U 73122 and
chelerythrine accordingly). 10 mM caffeine stimulated DNA synthesis in these cells, 1 μM U 73122 and 25 μM chelerythrine, on the contrary, blockades
the synthesis. 50 and 500 mM KCl, the agent which causes depolarization of plasma membrane stimulating Ca2+ entry into the cells, has no effect.
The transduction of the proliferative signals in T. pyriformis cells has been proposed to be of Ca2+-dependent nature.
Key words: Tetrahymena pyriformis, intracellular signaling, proliferation, EGF, caffeine, Ca2+, PLC and PKC
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