PULSED FIELD GEL ELECTROPHORESIS: THEORY, INSTRUMENTS AND APPLICATIONS
E. S. Nassonova
Institute of Cytology RAS, St. Petersburg;
e-mail: nosema@mail.ru
Pulsed Field Gel Electrophoresis (PFGE) is a powerful technique for the fractionation of high molecular weight DNAs ranging from 10 kb to 10 Mb in size. PFGE
separates DNA molecules in agarose gel by subjecting them to electric fields that alternate ("pulsate") in two directions. This technology plays a key role in the
modern genomics as it allows manipulations of the DNA of whole chromosomes or their large fragments. In this review we discuss: 1) the theory behind PFGE,
2) different instruments based on the principle of pulsed field, their advantages and limitations, 3) factors affecting the mobility of DNA in PFGE gels,
4) practical applications of the technique.
Key words: PFGE, DNA fractionation, chromosomal DNA
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