THE STUDY OF DNA INTEGRITY IN SOMATIC CELLS AND SPERMATOZOA BY SINGLE CELL GEL ELECTROPHORESIS
WITH SILVER STAINING
S. N. Proshin,1, * G. V. Stepanov,1 V. Yu. Kravtsov,1
G. P. Kosyakova,2 I. A. Paranyan,2 R. F. Fedortseva,1
A. F. Yakovlev 2
1 All-Russian Center for Emergency and Radiation Medicine and
2 All-Russian Institute for Farm Animal Genetics and Breeding, St. Petersburg;
* e-mail: psn@arcerm.spb.ru
In present work we studied DNA damage in human and bovine lymphocytes and spermatozoa by means of
single cell gel electrophoresis followed by silver staining. The spontaneous frequency of DNA damage estimated
manually in spermatozoa from healthy donors did not exceed 9 % (on average - 4.8 ± 1.2 %). The frequency
of DNA damages in bull sperm after short (less than a year) and long period (more than 20 years) of cryopreservation
was assessed as 3.1 ± 0.9 and 4.3 ± 0.5 %, correspondingly. The comparative estimation of DNA damages
in lymphocytes followed by silver staining is a valuable tool to estimate DNA damage in populations of
somatic and reproductive cells.
Key words: single cell gel electrophoresis, lymphocytes, spermatozoa, silver staining
Back
Contents
Main
|
