METHODOLOGICAL PROBLEMS OF DIGITAL CYTOPHOTOMETRY
G.I. Shtein,1 V.G. Panteleyev, B.N. Kudryavtsev
Institute of Cytology RAS, St. Petersburg;
1 e-mail: spbgistn@mail.ru
The article discusses the advantages and disadvantages of absorption cytophotometry and basic errors of
method. We examined the structure of modern equipment for cytophotometry studies with using of digital technologies.
In order to increase the accuracy of measurements it is offered some methods for determining glare,
dark current, non-uniformity of background and taking into account other digital cytophotometry errors. The
possibility of universal and specialized software for cytophotometry is discussed.
Key words:
cytophotometry, optical density, the digital image
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