THE EFFECT OF YEAST SACCHAROMYCES CEREVISIAE RED PIGMENT
ON THE EXPRESSION OF THE CLONED HUMAN α-SYNUCLEIN
O.V. Nevzglyadova, A.V. Artemov, E.V. Mikhailova, O.G. Lyublinskaya,
Yu.E. Ozerova, P.A. Ivanova, E.I. Kostyleva, T.R. Soidla 1
Institute of Cytology RAS, St. Petersburg, 194064;
1 e-mail: soidla39@gmail.com
Two transgenic yeast strains expressing human α-synuclein were used to study the effects of yeast red pigment
which, as we have shown earlier, exhibits antiamyloid properties. It has been demonstrated that endogenous
red pigment produced in special conditions by strains carrying ade1 mutation inhibits expression of the hybrid
protein α-synuclein-GFP. This is witnessed by a decrease in the mean value of GFP fluorescence and in the
per cent of cells exhibiting cytoplasmic inclusions of α-synuclein-GFP. Exogenous red pigment was used in a
form of purified natural pigment, its hydrolyzed derivative and a synthetic red pigment. All they differ from the
endogenous pigment by their effect on α-synuclein. Exogenous red pigments lead to increase of both the number
of cells expressing GFP fluorescence and the number of cytoplasmic inclusions. Both endogenous and exogenous
red pigment lead to drop of toxicity of the cloned a-synuclein and redistribution of the α-synuclein in
cells. The content of a-synuclein decreases in cell lysate pellets and increases in supernatants.
Key words:
amyloid, α-synuclein, red pigment, yeast
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