THE TARGET CELLS OF DIFFERENT LITHIUM FORMS IN HETEROGENEOUS POPULATION HEPATOCARCINOMA-29
Yu.S. Gavrilova,1 N.P. Bgatova, A.O. Solovieva, K.E. Trifonova, A.P. Lykov, Yu.I. Borodin, V.I. Konenkov
Scientific Institute of Ñlinical and Experimental Lymphology, Novosibirsk, 630060;
1 e-mail: inabrite@yandex.ru
Liver cancer is one of the most aggressive and heterogeneous human tumors. Lithium salts are block proliferation
and induce apoptosis of hepatocarcinoma cells but do not cause the death of the entire cell population.
The aim of the study was to identify the morphological types of target cells of various lithium forms at influence
on hepatocarcinoma-29. Using MTT test, the hepatocarcinoma-29 cells viability was determined and a dosedependent
decrease in the viability of the cells exposed to lithium carbonate, lithium citrate and nanosized
forms of lithium carbonate and lithium citrate were revealed. Target cells for lithium salts were identified on the
basis of morphological criteria of 5 stages of hepatocarcinoma-29 cells differentiation. It has been shown that
the target cells for lithium citrate of initial and nanosized forms of are proliferating cells of I and II stages of differentiation,
and the target cells for nanosized form of lithium carbonate are the differentiated cells at IV and
V stages of differentiation. It has been revealed that hepatocarcinoma-29 cells are most susceptible to the damage
effects of nanosized form of lithium salts in comparison to their original forms, which allows better impact
on the tumor growth.
Key words:
lithium salts, nanosized forms of lithium, hepatocarcinoma-29, target cells
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