NUCLEAR STRUCTURE IN THE SECRETORY CELLS OF MAMMARY GLANDS IN LACTATING AND NON-LACTATING RATS
K.V. Tyutina,1,2 V.G. Skopichev,2 D.S. Bogolyubov,1 I.O. Bogolyubova 1,3,*
1 Institute of Cytology RAS, St. Petersburg, 194064,
2 St. Petersburg State Academy of Veterinary Medicine, St. Petersburg, 196084, and
3 East European Institute of Psychoanalysis, St. Petersburg, 197198;
* e-mail: ibogol@mail.ru
The features of structural and functional organization of the main nuclear compartments and distribution of their key molecular components (chromatin-remodeling protein ATRX,
RNA polymerase I and II, and the splicing factor SC35) has been studied in the nuclei of mammary gland cells at different functional states. No significant differences between
the nuclei of the cells in the lactating and non-lactating mammary glands have been revealed at the ultrastructural level. At the same time, photometric analysis has revealed higher
intensity of nucleoplasmic immunofluorescent staining of mammary glands in the lactating animals when antibodies against the proteins ATRX and SC35 were used. Apparently, this
observation reflects the changes of the structural and functional status of chromatin as well as the redistribution of splicing factors between the sites of their deposition and
transcription.
Key words:
secretory cells of mammary gland, cell nucleus, nuclear domains, RNA polymerase I and II, chromatin-remodeling protein ATRX, SR-protein SC35
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