CHARACTERIZATION OF THE DIFFERENCE IN THE MORPHOLOGY OF DETERGENT
RESISTANT MEMBRANES DOMAINS ISOLATED FROM DIFFERENT CELL TYPES BY ATOMIC FORCE MICROSCOPY
S.N. Pleskova,1,2,* E.N. Aybeke,1 E. Bourillot,1 E. Lesniewska 1
1 Laboratoire Interdisciplinaire Carnot de Bourgogne, University de Bourgogne, Dijon, France, 21000,
and 2 N.I. Lobashevsky State University of Nizhniy Novgorod, Research
and Education Center "Physics of Solid State Nanostructure", Nizhniy Novgorod, Russia, 603950;
* e-mail: pleskova@mail.ru
Planar raft and caveolae are specific membrane clusters with high concentration of cholesterol and lipids with saturated fatty acid. These clasters are resistant to detergents and
are denoted as detergent resistant membranes domains (DRMs). Their morphology and size have been studied by atomic force microscopy. The size of planar rafts isolated by
Librol from monocytes of healthy volunteers was 150.6 ± 68.6 nm - diameters and 5.7 ± 2.9 nm - height, the size of caveolae was 87.3 ± 46.1 nm - diameters and 9.4 ± 5.4 nm - height.
Significant difference have been found morphology and size of DRMs isolated from monocytes of healthy volunteers and patients suffering from myocardial infarction as well as between
DRMs isolated from endothelial cells. The study of time-dependent changes in the morphology of isolated planar rafts and caveolae has shown that they quickly aggregate during
keeping. Therefore, to asses the actual size and morphology of the DRMS, they should be investigated immediately after isolation.
Key words:
planar raft, caveolae, morphology, size, atomic force microscopy
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