STIMULATION OF HaCaT KERATINOCYTES AND RATS MESENCHYMAL
STROMAL CELLS PROLIFERATION BY FEMTOSECOND LASER PULSES
A.N. Velikanov,1,* F.E. Gostev,2 E.A. Suprunenko,1 I.V. Shelaev,2
V.I. Yusupov,3 V.A. Nadtochenko 2
1 Biological Faculty of M.V. Lomonosov Moscow State University, Moscow, 199991,
2 N.N. Semenov Institute of Chemical Physics, RAS, Moscow, 119991,
and 3 Institute on Laser and Information Technologies RAS, Moscow, 140700;
* e-mail:av-bioem@mail.ru
The influence of femtosecond laser pulses on the proliferative activity of HaCaT keratinocytes and mesenchymal
stromal cells (MSC) rats was studied. The growth media was exposed by laser pulses with wavelength
590 nm and duration 30 fs. The dependence of proliferative activity of cells on the dose was showed in the range
6—4299 J/cm2. Proliferative activity was assessed by the number of cells after 1 day after exposure. For both
cell cultures obtained similar dose dependence: an increase in cell proliferation (32—54 % for HaCaT and 19 %
for MSK) occurs when using lower doses, while higher doses no changes the rate of proliferation of cells. Conducted
physical and chemical analysis found no increase in the concentration of active forms of oxygen in the
culture medium. The impact of femtosecond laser pulses has led to the generation in culture medium acoustic
oscillations in the range of 0.5 to 6.0 kHz. It is assumed that the increase in proliferative activity of cells, can be
caused by mechanical effects of acoustic waves generated in the environment of optical breakdown in the focus
of the laser radiation.
Key words:
laser pulses, photobiomodulation, reactive oxygen species, mechanobiology, stimulation of
proliferation, MSC, keratinocytes HaCaT
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