CELLS FORM AND THEIR SENSITIVITY TO LYTIC ACTIVITY OF NATURAL KILLER
CELLS UNDER THE ANTIOXIDANT ACTION
K.M. Kirpichnikova, Yu.P. Petrov, N.A. Filatova, I.A. Gamaley 1
Institute of Cytology RAS, St. Petersburg, 194064;
1 e-mail: igamaley@mail.cytspb.rssi.ru
The present paper is an attempt to estimate the influence of cell surface morphology changes to functional
activity under the effect of antioxidant, N-acetylcysteine (NAC), and alpha-lipoic asid (ALA). Two experimental
parameters were used to characterize transformed fibroblasts 3T3-SV40 status. The functional one was the
cell sensitivity to lysis by natural killer (NK) mouse splenocytes, and morphology index (cell form index) was a
cell area. We showed that addition of NAC or ALA to the cell medium caused fast decrease of cell area and
changes of cell form. On the other hand, their sensitivity to lysis NK cells gradually and significantly decreased.
Then we compared NAC or ALA effect with the effects of other substances, which were non-antioxidants but
caused cell responses which concurred with of antioxidants, at least partly. They were: latrunculin B, desorganizing
actin filaments (as both antioxidants), OTZ reducing ROS level in the cell (as NAC), BSO (inhibitor of
glutathione synthesis), increasing ROS level in the cell (as ALA), antibodies to gelatinases, MMP-2 and
MMP-9 inactivating their activities (as both antioxidants). The results obtained showed a correlation between
changes of morphology index and functional activity, sensitivity to lysis by NK cells. We suppose that geometry
of cell surface might be a functional indicator of cell reaction to the antioxidant.
Key words:
3T3-SV40 cells, surface morphology, natural killer cells, antioxidants NAC and ALA, latrunculin
B, OTZ, BSO, antibodies to MMP-2 and MMP-9
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