FUNCTIONAL STATE OF SCORPION FISH SCORPAENA PORCUS L. NUCLEATED ERYTHROCYTES UNDER DOSED HYPOXIA
À.Yu. Àndreyeva,1 À.À. Soldatov, V.S. Mukhanov
Institute of Marine Biological Research Named after A.O. Kovalevsky RAS, 299011;
1 e-mail: andreevaal@gmail.com
Hypoxia is a wide spread phenomena throughout the world’s aquatic systems. This paper investigates the
functional state of scorpion fish red blood cells under acute hypoxia between 0.57—8.17 mg Î2/l (in vitro) by
assessing of the fluorescence of Rhodamine 123 (R123) and Fluorescein diacetate (FDA). Acute hypoxia induced
a significant increase in the fluorescence of both fluorochromes (R123 — 12—60, FDA — 30—184 %).
This reaction is based on a probable decrease in erythrocyte membrane permeability. The reduction of functional
ionic channels in red blood cells delays FDA in the cytoplasm and lowers the energy costs required for
transmembrane cationic gradient maintenance, which reflects an intensification of R123 fluorescence.
Key words:
red blood cells, flow cytometry, hypoxia, Scorpaena porcus L., R123, FDA
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