COMPARATIVE CYTOGENETIC ANALYSIS OF MONOLAYER AND SUSPENSION CHINESE HAMSTER OVARY CELL LINES CHOdhfr–
V.N. Stefanova,1,* N.M. Yartseva,1 A.V. Petrov 2
1 Institute of Cytology RAS, St. Petersburg, 194064, and 2 Institute of Highly Pure Biopreparations, St. Petersburg, 197110;
* e-mail: vestefan@mail.ru
The karyotypes of CHOdhfr– and CHOdhfr–/susp Chinese hamster ovary cell lines were investigated with the
use of GTG-staining. Modal chromosome set consists of 20 and 18 chromosomes respectively. The karyotypes
of both cell lines were stable with constant ratio of normal chromosomes and chromosomes with structural rearrangements.
Monosomy for chromosomes 1, 2, 4, 5, 8 was observed in both cell lines and for chromosome 9 in
CHOdhfr–/susp cell line. The differences between CHOdhfr– cell lines studied by us consists of inclusion of part
of chromosome 7 in der(6)t(1;6), rearrangement of del(5) and monosomy of chromosome 9. It was shown that
in karyotypes of all CHO cell lines studied up today there are 5 common structurally chromosome rearrangements:
del(2), inv(3), add(6), del(9) and mar1. In both CHOdhfr– cell lines investigated by us three unique chromosome
rearrangements: del(1), der(6)t(1,6) and mar3 were revealed. Necessity of simultaneous GTG and
FISH analysis of chromosomes rearrangements in the CHO cell lines under study is discussed.
Key words:
CHO cell lines, karyotype, marker chromosomes, chromosome rearrangements
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