IMPACT OF VARIOUS MULTIPLICITY OF INFECTION OF INFLUENZA A VIRUS ON PROLIFERATION AND APOPTOSIS INDUCTION IN CULTURED
CELL LINES OF LYMPHOCYTIC AND MONOCYTIC ORIGIN (JURKAT, NC-37, THP-1 AND U-937)
T.D. Smirnova, D.M. Danilenko, E.V. Ilyinskaya, S.S. Smirnova, M.Yu. Eropkin
Research Institute of Influenza, Ministry of Health of Russian Federation, St. Petersburg, 197022;
e-mail: cellcultures@influenza.spb.ru
The severity of disease caused by influenza A infection depends not only on biological characteristics of
the virus but also on the number of viral particles than penetrate the body. T- and B-lymphocytes as well as monocytes
(macrophages) play a key role in the development of cell-based and humoral immunity as well as influenza
virus elimination from the body. The present study describes the effect of influenza A virus infection on
cell proliferation and induction of apoptosis in human cultured cell lines of T-, B-lymphocytic and monocytic
origin infected with various multiplicity of infection (moi). Low moi of the virus stimulated cell proliferation;
maximal effect has been registered 3—4 days after infection. But the fate of T-cells, B-cells and monocytes after
initial infection was different: Jurkat cells continued intense proliferation while proliferation of NC-37,
THP-1 and U-937 cells lowered. Prolonged (for 3 passages) cultivation of Jurkat, NC-37 and U-937 cell lines
has shown that infection of these cell lines not only with low but also with medium and high moi also leads to
stimulation of proliferation. Using a variety of methods for the detection of viral reproduction has clearly
shown that infection of non-permissive human T-, B-cells and monocytes with influenza A virus leads to latent
infection. So, low moi interferes with normal formation of viral particles, which in turn might stimulate cell
proliferation and then be followed by induction of apoptosis. Antiviral drags rimantadine and ribavirin suppressed
virus-induced cell proliferation; at the same time, induction of apoptosis was suppressed only by rimantadine
and was enhanced by ribavirin. The data obtained provide strong support for the role of influenza A virus in
the observed effects.
Key words:
T- and B-lymphoblastoid and monocytic cell lines, influenza A virus, cell proliferation, apoptosis, multiplicity of infection
| Back
| Contents
| Main |
|
