THE EFFECT OF EPOXYGENASE INHIBITORS ON Ca2+-RESPONSES INDUCED BY GLUTOXIM AND MOLIXAN IN MACROPHAGES
L.S. Milenina, Z.I. Krutetskaya, A.A. Naumova, S.N. Butov, N.I. Krutetskaya, V.G. Antonov
Chair of Biophysics, St. Petersburg State University, 199034;
e-mail:cozzy@mail.ru
Using Fura-2AM microfluorimetry the possible involvement of epoxygenase pathway of arachidonic acid
metabolism in the effect of glutoxim and molixan on intracellular Ca2+ concentration in rat peritoneal macrophages
was investigated. It was shown for the first time that preincubation of the macrophages with epoxygenase
inhibitors, proadifen and econazole, significantly decreases the intracellular Ca2+ concentration increase
induced by glutoxim and molixan. The addition of the epoxygenase inhibitors during the already developed store-
dependent Ca2+-entry induced by glutoxim or molixan partially inhibits Ca2+-entry. The obtained data suggest
the involvement of the products and/or enzymes of epoxygenase pathway of the arachidonic acid metabolism
in the glutoxim and molixan effect on the Ca2+ signaling processes in macrophages.
Key words:
intracellular Ca2+ concentration, peritoneal macrophages, glutoxim, molixan, arachidonic acid, epoxygenases
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