EFFECT OF STROMAL CELLS AND OXYGEN CONCENTRATION ON THE MAINTENANCE
OF CORD BLOOD HEMATOPOIETIC PRECURSORS
E V. Sotnezova (Maslova),1 A.N. Gornostaeva,1 E.R. Andreeva,1 Yu. A. Romanov,2
E.E. Balashova,2 L.B. Buravkova 1,*
1 Institute of Biomedical Problems RAS, Moscow, 123007,
and 2 National Complex of Cardiology Research and Practice, Ministry of Health of the Russian Federation, Moscow, 121552;
* e-mail: buravkova@imbp.ru
The paper analyses the morphological and functional features of cord blood cells (CBCs) in the co-culture
with multipotent mesenchymal stromal cells (MMSCs) from human adipose tissue under tissue-related oxygen.
We have established that MMSCs effectively maintained viability of CBCs at different oxygen concentrations
(1, 5 and 20 %). According to the data obtained, the oxygen concentration affected the number of colony-forming
units (CFUs) formed by CBCs in selective medium. In particular, not co-cultured CBCs the 1 and 5 % O2
than at 20 % O2. After co-culture with MMSCs, the CFUs numbers were similar at 1 and 20 % O2 and increased
by 30 at 5 % O2. Tissue related O2 concentrations had an impact on the proportion of lineage-resctricted CFCs
among CBCs: the number of more committed progenitors — CFU-G and CFU-M, increased, and multi and bipotent
— CFU-GEMM and CFU-GM, decreased at low oxygen concentrations. This effect was more pronounced
after co-culture compared to that of initial CBCs. Thus, the presence of stromal cells and tissue-related oxygen
jointly and severally influenced CBCs in vitro.
Key words:
cord blood, hematopoietic stem cells, multipotient mesenchymal stromal cells, co-culture, oxygen
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