PECULIAR PROPERTIES OF SOME COMPONENTS OF MORPHOLOGICAL STRUCTURE
IN A PLANT CELL VACUOLE REVEALED BY CONFOCAL MICROSCOPY
V.N. Nurminsky,1,* A.L. Rakevich,2 E.F. Martynovich,2 N.V. Ozolina,1 I.S. Nesterkina,1
E.V. Kolesnikova,1 A.A. Pilipchenko,3 R.K. Salyaev,1 M.Yu. Chernyshov 4
1 Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch of RAS, Irkutsk, 664033,
2 Institute of Laser Physics (Irkutsk Department), Siberian Branch of RAS, 664003,
3 Irkutsk State University, 664033, and
4 Presidium of Irkutsk Scientific Center, Siberian Branch of RAS, 664033;
* e-mail: cell@sifibr.irk.ru
Results of investigations of peculiar properties related to the structure of plant cell vacuolar membranes are
discussed. The study was carried out using confocal microscopy, which allowed us in the process of scanning to
identify membrane tubes and vesicules in the preparations of isolated vacuoles. Such membrane tubes were found
both inside and outside the vacuoles, and, in the case of scanning intermittently at equal time intervals,
transition of vesicles with the membrane tube was observed. Furthermore, scanning of isolated vacuoles was
conducted at various distances from the glass substrate. Each time, in the upper area of the isolated vacuole
lying on the substrate, we observed a large segment of vacuolar membrane and registered the effect of highly intensive
fluorescing of some of membrane segments. The distributions of laurdan fluorescence generalized polarization
(GP) values for the vacuolar membrane on the whole and for the intensively fluorescing membrane segments
have been obtained. We have found that the microviscosity of the intensively fluorescing membrane
segments essentially differs from that of the rest part of the membrane.
Key words:
plant cell vacuoles, structure of vacuolar membrane, confocal microscopy, fluorescent probes,
membrane tubes, microviscosity of membrane
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