CYTOGENETIC RESPONSE OF SCOTS PINE (PINUS SYLVESTRIS L.) TO CADMIUM AND NICKEL
M.V. Belousov,1,* O.S. Mashkina 2
1 St. Petersburg State University, 199034, and 2 Voronezh State University, 394006;
* e-mail: belousovmix@gmail.com
We studied cytogenetic polymorphism of the seeds of Pinus sylvestris L. in response to heavy metals exposure
in laboratory settings over 2 years’ time. We compared results obtained from the seedlings of different
years: 2012 and 2013. With an increase in Ni2+ and Cd2+ concentration we observed a decrease in mitotic activity
with concurrent rise in the percentage of cells in the prophase. This fact demonstrates the heavy metals act similar
to both fixatives and substances that block cleavage spindle formation. In terms of pathological mitosis
and the frequency of micronuclei cells, Cd2+ shows higher mutagenity compared to Ni2+. In addition, in the experimental
samples, we have distinguished abnormalities such as fragmentations and agglutinations of chromosomes
and especially C mitosis occurrence, which are not observed in the control.
Key words:
cadmium, C mitosis, micronuclei, mitotic activity, nickel, mitosis abnormalities, Scots pine,heavy metals
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