SPREADING OF NCTC CLON 929 CELLS AFTER RESEEDING
Yu.P. Petrov,1,* Yu.A. Negulyaev,1,2 N.V. Tsupkina 1
1Institute of Cytology RAS, St. Petersburg, and
1 Department of Medical Physics, St. Petersburg State Polytechnical University;
* e-mail: yupe3ov@mail.ru
The period (1 h after reseeding) of behaviour of mouse NCTC clone 929 cells to the conditions of artificial
cultivation was studied. The time-lapse imaging followed the processing of the cells with ImageJ program was
applied. To characterize the parametres cell status we used the cell area (projection of the cell on substrate) and
Rp/Ra ratio introduced earlier as a spreading coefficient (Kuz’minykh, Petrov, 2004). After attaching a substratum,
cells have a form of sphere (the phase «sphere») as the daughter cells after a mitosis. We revealed however
that after this phase the reseeded cells do not start usual spreading and migration along substratum. They pass a
phase of equally spreading in all directions and shaping their area as a circle (phase «circle»). This phase is absent
of the daughter cells spreading after mitosis. We assume that the phase «circle» is a result of adaptation of
the cells to reseedings at artificial cultivation. It is necessary for formation of a substrate composed of own extracellular
matrix components (ECM) of the cells. Own ECM facilitates transition of the cells to their usual spreading
and migration along substratum.
Key words:
adhesion, spreading, cell morphometry, mitosis, ECM, filopodium
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