FEATURES OF tRNA GENES AND YOUNG SUBFAMILIES Alu-REPEATS EXPRESSION
IN PROLIFERATION AND APOPTOSIS
I.N. Kabanov,1 L.I. Tishchenko
Department of Biochemistry of St. Petersburg State University;
1 e-mail: Ink5@yandex.ru
Using human U937 cell culture, we studied the expression of the RNA polymerase III-directed genes —
tRNAiMet1, tRNAHis and Alu-repeats belonging to the youngest subfamilies AluY (AluYa5 and AluYb8)—during
camptothecin-induced apoptosis. The level of tRNAiMet1 increased 1.5-fold, tRNAHis level did not change,
and the level of AluY-RNA increased 4—10-fold after 6 hours of CAM treatment compared to control
(non-apoptotic) cells. We also studied the level of AluYb8 DNA methylation at apoptosis U937. We have
shown that the level of AluYb8 DNA methylation does not change at different stages of apoptosis, and does not
differ in apoptotic and control cells. We assume that the increase in gene expression of young AluY repeats and
tRNAiMet1 plays a role in the apoptosis pathway realization in the cell.
Key words:
class III genes, RNA polymerase III, stable non-translated RNAs, SINE, Alu-repeats, real-
time RT-PCR, DNA methylation, apoptosis
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