NUCLEOSOME FRACTON OF EXTRACELLULAR DNA AS THE INDEX OF APOPTOSIS
I.N. Vasilyeva,1,* Î.I. Podgornaya,2 V.G. Bespalov 1
1 N.N. Petrov Research Institute of Oncology and 2 Institute of Cytology RAS, St. Petersburg;
* e-mail: iravasilyeva@hotmail.com
Review is devoted to the analysis of changes of the extracellular DNA (excDNA) in pathological conditions
involving the process of apoptosis, and the possibility of using of excDNA in the diagnosis and evaluation
of course of various diseases. Apoptosis is the main mechanism of the appearance of the DNA in the circulation.
ExcDNA found in the norm, its function is considered to be the part of the immune response. The excDNA
content increases substantially during the induction of apoptosis. Dynamics of increase of excDNA content in
stroke allows to diagnose the form of stroke and massiveness of destruction of brain tissue. Reduced content of
excDNA is associated with the inhibition of apoptosis, it was shown that under such conditions there is a change
of composition of excDNA. Investigation of excDNA character changes in the progression and treatment of
cancer substantiates the possibility of early assessment the effectiveness of treatment. It is experimentally
shown the immunosuppressive action of excDNA of tumor and its transforming effect on the cells. Ionizing radiation
is an examples that demonstrated the association of induction of apoptosis and the release of excDNA. It
is characterize some of the genome sequences of excDNA. Created on the basis of excDNA study tests of minimally
invasive diagnostics are potentially useful in oncology and other areas of medicine. The study of tandem
repeats, which are absent in the assembled genome, but there is a part of excDNA, will create tests for the diagnosis
of cancer in the early stages.
Key words:
extracellular DNA, apoptosis, nucleosoms, cancer, stroke, minimum invasive diagnostics
| Back
| Contents
| Main |
