ŃULTURE OF MUSSEL MYTIULS EDULIS L. MANTLE CELLS
M.A. Daugavet,1 M.I. Blinova
Institute of Cytology RAS, St. Petersburg;
1 e-mail: ka6tanka@yandex.ru
To date, cell lines derived from marine invertebrates have not been available. Hence primary cell cultures
serve as model systems for various experiments. In present study we established primary culture of mussel Mytilus
edulis L. mantle cells. Cells were isolated by means of explant culture or enzymatic dissociation of mantle
tissue. They maintained viability up to 22 months regardless of culture initiation method. In course of culturing,
cells, which were transferred onto new plates, successfully attached to a new surface. Physiological activity of
cultured cells was also confirmed by formation of crystals, which appeared after 4—6 months. After continuous
time of culturing, mantle cells can be cryopreserved using 5 % DMSO with post-freezing survival up to 50 %.
These results demonstrate that M. edulis mantle cells can maintain viability and physiological activity for exceptionally
long time and can be cryopreserved for further examination.
Key words:
marine invertebrates, mollusks, mantel, cell culture, cryconservation
| Back
| Contents
| Main |
