THE STUDY OF THE ORIGIN OF HUMAN GLIOMA BASED ON CELL LINES AND TUMOR SPECIMENS
G.V. Timin,1 Yu.S. Lakhina,2 D.A. Gulyaev,2 E.N. Tolkunova 1,*
1 Institute of Cytology RAS, St. Petersburg, 194064,
and 2 Polenov Russian Scientific Research Institute of Neurosurgery
(part of Federal V.A. Almazov North-West Medical Research Centre), St. Petersburg, 191014;
* e-mail: bay@entolk62@mail.ru
The present study is dedicated to the search of human glioma "cells of origin". Specimens of the tumor tissue have been analyzed via the RT-PCR method to potentially reveal the
expression of molecular markers characteristic of various cells of nerve tissue (NeuN, MOG, MBP, NG2, Olig2, Vimentin, GFAP, Aldh1L1), as well as markers of stem (Oct4, c-Kit) and
cancerous stem (CD133) cells. We have shown that the expression profiles of these markers for different types of gliomas intersect, which does not allow to determine the type of
"cells of origin". So, in order to study the origin of glioma using cell lines derived from primary cultures, we need a more sophisticated culture conditions, rather than the commonly used
serum-based media.
Key words:
glioma, cancer stem cells, cells of origin
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