PAIRWISE CROSS-SPECIES TRANSCRIPTOME ANALYSIS OF POLYPLOIDY-ASSOCIATED EXPRESSION CHANGES OF DEVELOPMENTAL GENE MODULES
O.V. Anatskaya,1,* E.A. Erenpreisa,2 N.N. Nikolsky,1 A.E. Vinogradov 1
1 Institute of Cytology RAS, St. Petersburg, 194064,
and 2 Latvian Biomedical Research and Study Centre, Riga, LV-1067, Latvia;
* e-mail: olga.anatskaya@gmail.com
Design and development of highly sensitive method bioinformatics are important for investigation of casual relationships between epigenetic changes and gene activity.
Cell polyploidy may trigger such changes. However, maintaining the balance of gene dosage, polyploidy may provide only a rather weak effect on their expression. Currently,
there is no comprehensive and concordant data in regard to ploidy-associated transcriptomic changes. To find out how polypoidy affects gene activity, we have developed an
integrative bioinformatic method of pairwise cross-species transcriptome analysis of mammalian tissues with various polyploidy degrees. The main benefit of this approach is its
ability to separate species- and tissue-specific noises of evolutionary conserved effects. We demonstrat the application of the method for the analysis of gene modules and protein
interactions networks coordinating programs of development, differentiation and pluripotency. The analysis was performed with transcriptomes of polyploid and diploid organs
(human and mouse heart and liver). Our data indicate that ploidy-induced genes enrich Gene Ontology (GO) biological processes and KEGG pathways related to development,
morphogenesis and stem cells biology (including Hippo, Pi3K, WNT, Hedgehog and TGF- b pathways) with higher degree than ploidy-inhibited genes. Thas, our data are the first to
show that polyploidy may induce and coordinate developmental modules.
Key words:
comparative transcriptome analysis, protein interaction networks, gene modules, polyploidy, development, differentiation, stem cell, fetal and embryonic phenotype
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