CHARACTERISTIC OF ENDOMETRIAL MESENCHYMAL STEM CELLS IN CULTURE OBTAINED FROM PATIENT WITH ADENOMYOSIS
M.A. Shilina,1 A.P. Domnina, I.V. Kozhukharova, V.V. Zenin, S.V. Anisimov, N.N. Nikolsky, T.M. Grinchuk
Institute of Cytology RAS, St. Petersburg, 194064;
1 e-mail: shili-mariya@yandex.ru
Adenomyosis is form of endometriosis, common diseases of female reproductive system, which can lead to infertility in women. In this study we are obtained and characterized
cell line endometrial mesenchymal stem cells from a patient with adenomyosis, and compare obtained cells with the cell line of healthy donor. Aim of this study was to assesses
the extent of differences between cells from donor with adenomyosis and cells from healthy donor. Was established that compared lines had morphology like fibroblasts, were
differentiated in adi-pocytes, were expressed mesenchymal markers and didn't expressed haematopoietic markers. Cytogenetic analysis of differentially stained metaphase
chromosomes on G-banding (passage 6-7) showed that healthy donor's cells had predominantly normal karyotype. The cellular line from a patient with diagnosis of " adenomyosis"
had a lot of cells with changes in karyotype's structure. These changes were related with aneuploidy of cellular population and the presence non-random chromosomal breaks, often
in chromosomes 7 and 11. Analysis of this data allows the cells from adenomyosis characterized physiological stability in culture and karyotypic instability with non-random involvement
certain chromosomal set. The cellular line obtained from donor with adenomyosis showed signs destabilization of he genome, typical for cell transformation. Division of adenomyosis
cells to the 26th passage is stopped and these cells entered into a phase of replicative aging. Based on this, we can conclude that founded karyotype's hanges do not lead to
transformation and immortalization of cells in vitro.
Key words:
endometrial mesenchymal stem cells, adenomyosis, endometriosis, karyotype, chromosomal changes, chromosomal breakage
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