FUNCTIONAL LINK BETWEEN TRANSPORT PARAMETERS OF MDCK1 CELLS MONOLAYER AND THEIR ACTIN CYTOSKELETON ORGANIZATION
A.N. Gorshkov,1,2,* M.R. Zaitzeva,1,3 E.S. Snigirevskaya,1 Ya.Yu. Komissarchik 1,3
1 Institute of Cytology RAS, St. Petersburg, 194064,
2 FSBI Research Institute of Influenza Ministry of Healthcare of the Russian Federation, St. Petersburg, 197376,
and 3 St. Petersburg State University, Faculty of Biology, 199034;
* e-mail: angorsh@yahoo.com
On MDCK1 cell monolayer, dynamics of the spatial organization of actin cytoskeleton and dynamics of trans-epithelial electrical resistance (TEER) have been studied upon
exposure of arginine-vasopressin (AVP) and protein-kinase A (PKA) activator forskolin. It has been found that exposure to these physiologically active compounds causes fibrillary
actin depolymerization (both in apical and in basal cytoplasm) and, simultaneously, significant decrease in the cell monolayer trans-epithelial electrical resistance. TEER decrease
indicates the stimulation of ions and water flow across the cell monolayer. In order to clarify pathways of movement of ions and water across MDCK monolayer, we have carried out an
immunofluoresence study of claudin 1 and 2 localization in the tight junctions of MDCK ATCC cells (low TEER) and MDCK1 cells (high TEER). We have demonstrated that in the tight
junctions of MDCK ATCC cells both claudin 1 and claudin 2 are present. In MDCK1 cells tight junctions, claudin 1 is localized and pore-forming claudin 2 is completely lacking. Under
forskolin exposure to MDCK1 cells, no alterations in studied claudins distribution has been found. These data indicate that forskolin-induced TEER decrease is linked with alterations
in trans-cellular, not in para-cellular, permeability of monolayer.
Key words:
arginine-vasopressin, MDCK cells, trans-epithelial electrical resistance, action cytoskeleton
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