THE USE OF THE COMMERCIAL APPARATUS DUAL GEL MODULE FOR THE TWO-DIMENSIONAL POLYACRYLAMIDE GEL ELECTROPHORESIS
I.N. Evteeva,1 T.Yu. Starkova, A.V. Artemov, N.A. Barlev 2
Institute of Cytology RAS, St. Petersburg, 194064;
e-mail: 1 evtin@mail.cytspb.rssi.ru,
2 nick.a.barlev@gmail.com
Two-dimensional gel electrophoresis, continues to be one of the fundamental methods to study the biological protein diversity. This method described by O'Farrell in 1975 includes
two following steps: isoelectric focusing in the first dimension and polyacrylamide gel electrophoretic fractionation of proteins according to their molecular weight in the second
dimension. In this manuscript we described several technical parameters of the commercial apparatus Dual Gel Module for the gel electrophoresis by means of which it is possible
to accomplish the electrophoretic protein fractionation in both dimensions. The distribution of the highly purified commercial proteins used as molecular standards in the detection
system of the apparatus Dual Gel Module was identical to the commercial strips of the device GE Healthcare, USA.
Key words:
two-dimensional gel electrophoresis, isoelectric focusing, strips, commercial modul, ampholines
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