Tsitologiya  2015  57 (10) : 727–735

EFFECT OF FUCOIDANS ISOLATED FROM SEAWEEDS LAMINARIA DIGITATА AND FUCUS VESICULOSUS ON CELL LINES HELA G-63, ECV 304 AND PC 12 E.V. Zhurishkina,1 I.M. Lapina,1 D.R. Ivanen,1 S.I. Stepanov,1 S.V. Shvetsova,1,2 A.L. Shavarda,3 N.Ya. Giliano,1 A.A. Kulminskaya 1,2 1 National Research Center "Kurchatov Institute", B. P. Konstantinov Petersburg Nuclear Physics Institute, Gatchina, 188300, 2 St. Petersburg State Polytechnical University, 195251, and 3 Research Resource Centre for Molecular and Cell Technologies, St. Petersburg State University, 199034; 1,2 e-mail: kulm@omrb.pnpi.spb.ru The aim of the research was to investigate cytotoxicity of fucoidans on mammals cells. Three different samples of fucoidans were isolated from mechanically grounded brown algae Laminaria digitata and Fucus vesiculosus. The sample F2 that differed from the others by higher sulfatation level and suppression of HeLa G-63 line culture growth was taken for further study in cell lines HeLa G-63, ECV 304 and PC 12. We have shown that fucoidan preparation F2 inhibits proliferation and induces cell death in a dose- and time-dependent manner for all investigated cell lines. Neuroendocrine tumor rat cell line PC 12 appeared to be the most sensitive to fu-coidan treatment whereas endothelial human cells ECV 304 were the least sensitive. Key words:  fucoidan, proliferation, flow cytometry |  Back   |  Contents   |  Home EN  |