OXIDATIVE STRESS-PROMOTED RESPONSES IN HUMAN ENDOMETRIAL STEM CELLS AND LUNG EMBRYONIC FIBROBLASTS
A.N. Shatrova,1 O.G. Lyublinskaya, A.V. Borodkina, E.B. Burova
Institute of Cytology RAS, St. Petersburg;
1 e-mail: Shatrova@mail.ru
Human mesenchymal stem cells are an attractive cell source for tissue engineering. During transplantation
they may be subjected to oxidative stress due to unfavorable cellular microenvironment, which is characterized
by increased levels of reactive oxygen species. Recently, we have demonstrated that oxidative stress responses
of human mesenchymal stem cells derived from endometrium (hMESCs) depend upon the oxidizer concentration.
Besides, the duration of the cell treatment with oxidizer may play an important role. In this study, we investigate
dependence of the cell response character on Í2Î2-treatment duration. The effects of the high Í2Î2 doses
on hMESCs and human lung embryonic fibroblasts were compared. In both cell types, Í2Î2-treatment for
60 min was shown to promote the multiphase cell cycle arrest, as well as to the dose-dependent cell death that
occurred equally from all phases of cell cycle. However, the cell death dynamics in hMESCs and fibroblasts
were different. Interestingly, in both cell types, shortening of Í2Î2-treatment duration from 60 to 10 min induced
growth retardation, G1-phase accumulation and the cell size increase. Together, these findings allow us to
suggest an induction of the premature senescence as a result of the short cell exposure to the high Í2Î2 doses.
Thus, regarding both human endometrial stem cells and human embryonic fibroblasts, shortening of oxidative
stress duration induced by high Í2Î2 doses enables to avoid the cell death and to produce the features of the
premature senescence.
Key words:
urokinase, urokinase receptor, angiogenesis, guidance receptors, vessel sprouting
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