EXPRESSION OF THE LIGHT CHAINS OF IMMUNOGLOBULINS IN NORMAL B-CELLS AND SOME B-CELL LYMPHOMAS
O.A. Khudoleeva,1 I.A. Vorobjev 1, 2, *
1 A.N. Belozersky Institute of Physico-Chemical Biology
and 2 Biological Faculty M.V. Lomonosov Moscow State University, 119991;
* e-mail: ivorobjev@mail.ru
The quantitative method of determining the level of expression of immunoglobulin light chains on uncompensated
data was suggested and used to examine disorders in light chain expression in various B-cell tumors.
The average level of expression of the lambda isotype was 4 times higher than the level of expression of kappa
isotype. The level of surface and cytoplasmic expression of LC IG varied within wide limits for different people,
but there was a high degree of correlation between the levels of expression of kappa and lambda isotypes LC
IG as well as between expression of the surface and cytoplasmic forms of each in isotype the same individual.
In the majority of B-cell non-Hodgkin’s lymphomas correlation between the expression of LC IG on the surface
and in the cytoplasm of the cells was diminished. Expression of LC IG in CLL was significantly reduced on the
surface of the cells and to a lesser extent — in the cytoplasm. In the case of marginal zone cell lymphoma, LC
IG expression level was reduced on the surface of circulating cells and to a lesser extent — in the cytoplasm. In
the case of mantle cell lymphoma and DLBCL, expression level of LC IG on the cell surface and in the cytoplasm
was the same as in.
Key words:
B-cells, lymphoma, immunoglobulin light chains, flow cytometry, antigen expression
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