THE MODEL OF NEUROVASCULAR UNIT IN VITRO CONSISTING OF THREE CELLS TYPES
E.D. Khilazheva,1 E.B. Boytsova, E.A. Pozhilenkova, Yu.R. Solonchuk, A.B. Salmina
Krasnoyarsk State Medical University named after prof. V.F. Voino-Yasenetsky, Krasnoyarsk, 660022;
1 e-mail: elena.hilazheva@mail.ru
There are many ways to model blood brain barrier and neurovascular unit in vitro. All existing models have
their disadvantages, advantages and some peculiarities of preparation and usage. We obtained the three-cells
neurovascular unit model in vitro using progenitor cells isolated from the rat embryos brain (Wistar, 14—16 d).
After withdrawal of the progenitor cells the neurospheres were cultured with subsequent differentiation into astrocytes
and neurons. Endothelial cells were isolated from embryonic brain too. During the differentiation of
progenitor cells the astrocytes monolayer formation occurs after 7—9 d, neurons monolayer — after 10—14 d,
endothelial cells monolayer—after 7 d. Our protocol for simultaneous isolation and cultivation of neurons, astrocytes
and endothelial cells reduces the time needed to obtain neurovascular unit model in vitro, consisting of
three cells types and reduce the number of animals used. It is also important to note the cerebral origin of all cell
types, which is also an advantage of our model in vitro.
Key words:
neurovascular unit, blood-brain barrier, model in vitro, progenitor cells, co-culture
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