COHESION INSIDE EU- AND HETEROCHROMATIN IN HUMAN CELLS
V.D. Cherepanynets,1,2,* O.A. Zhironkina,1,2 O.S. Strelkova,2,3
S.Yu. Kurchashova,2 I.I. Kireev 1,2
1 Faculty of Biology, M.V. Lomonosov Moscow State University, 2 A.N. Belozersky Institute of Physical
and Chemical Biology, M.V. Lomonosov Moscow State University, and 3 Faculty of Bioengineering and Bioinformatics,
M.V. Lomonosov Moscow State University;
* e-mail: temchromatinlab@gmail.com
It is considered that sister chromatids are held together immediately after replication by special protein
complex — cohesin that consists of Smc1—Smc3 core dimer and two additional subunits, Scc1 and Scc3. This
process is called cohesion. We have characterized binding of cohesin complex to early- and late-replicated
chromatin at different stages of the cell cycle in human cells HeLa and HT1080 using superresolution microscopy
(based on Structural ilumination microscopy—SIM) and immunoelectron microscopy. It has been shown
that cohesins do not play important role in cohesion of heterochromatic domains, but they provide cohesion and
organization of subdomains in euchromatic regions.
Key words:
cohesin, chromatin, euchromatin, heterochromatin, segregation, sister chromatids, superresolution
microscopy, immunoelectron microscopy
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