CHANGE OF EXPRESSION OF INTEGRINS ON MULTIPOTENT MESENCHYMAL STROMAL
CELLS ISOLATED FROM HUMAN ADIPOSE TISSUE DURING LONG CULTIVATION
I.P. Savchenkova,1 E.A. Savchenkovŕ
All-Russian State Research Institute of Experimental Veterinary Medicine of Ya.R. Kovalenko, Moscow;
1 e-mail: s-ip@mail.ru
An expression of integrins on multipotent mesenchymal stromal cells (MMSCs), isolated from human subcutaneous
adipose tissue during long cultivation was studied. Results of the comparative analysis of MMSCs on
the 2nd and 17th passages of cultivation revealed considerable distinctions in expression of α1, α4 and α6 integrins
on these cells. Strong decrease for 87.2 and 11.2 %, quantities of the cells which have been positively
stained by AT against CD49a (α1 integrin) and CD49d (α4 integrin), respectively was observed. The share of
the cells which have been positively stained by AT against CD49f (α6 integrin) and CD49b (α2 integrin) on the
contrary was increased during long cultivation by 9.9 and 2.3 %, respectively. The high expression of b1 integrin
(98 %) on MMSCs, wasn’t changed significantly in the course of long cultivation. As a result of induction
of MMSCs to a differentiation in the osteogenic direction it was revealed that cells on the 17th passage of cultivation
concede by efficiency of formation of cells of bone tissue and extracellular matrix in vitro to cells which
induced on the 2nd passage. Thus, long cultivation of MMSCs which is required for building of cellular population
after isolation, influences on a cytoskeleton and adhesive abilities of cells that it is necessary to consider
when using them for cellular technologies, including for modeling of this or that tissue in three-dimensional
scaffolds.
Key words: multipotent mesenchymal stromal cells, subcutaneous adipose tissue, human, integrins,
gene expression, differentiation, long cultivation
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