MODULATION OF APOPTOSIS IN MONONUCLEAR CELLS WITH DIFFERENT VARIANTS
OF POLYMORPHISM C-262T IN CATALASE GENE
A.V. Komina,1 T.G. Ruksha
Krasnoyarsk State Medical University;
1 e-mail: komivlann@yandex.ru
Catalase is a clue enzyme metabolizing hydrogen peroxide under conditions of oxidative stress. It is known
that the C-262T polymorphism in catalase gene is implicated in higher risk of some diseases onset. In this article
we have evaluated the viability of mononuclear leukocytes isolated from patients with different variants of
C-262T polymorphism in catalase gene (CC, CT, TT) under conditions of hydrogen peroxide induced oxidative
stress. It has been revealed that cells with TT variant of polymorphism have lower viability in the presence of
hydrogen peroxide in a concentration of 1000 μM/L in comparison with cells having CC and CT genotypes.
Furthermore, unlike CC and CT variants, cells with TT polymorphism showed no activation of mitochondrial
apoptotic pathway even with hydrogen peroxide at a concentration of 1 mM. The data obtained may explain the
increased risk of diseases related with the activity of the antioxidant system in patients with 262 TT promoter
polymorphism in catalase gene.
Key words: oxidative stress, hydrogen peroxide, apoptosis, TSPO
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