STRESS PROTEINS IN THE CELLS OF PORPHYRA PURPUREA (RHODOPHYTA) THALLUS
Yu.I. Podlipaeva,1,* S. Fulda,2 A.V. Goodkov 1
1 Institute of Cytology RAS, St. Petersburg, Russia, and 2 University of Rostock, Institute of Biosciences, Germany;
* e-mail: podlipaeva@gmail.com
Heat shock proteins have been revealed for the first time by the methods of Western blotting using alkaline
phosphatase and ECL in the cells of Porphyra purpurea from Kattegat area of the Baltic Sea in normal and experimental
stress conditions. It was demonstrated with application of monoclonal anti-Hsp70 antibodies that a
slight band about 70 kDa is present constitutively at the film; additionally the polypeptide of about 40 kDa
(«Hsp40») has been detected. After heat shock at 28 °C during 1 hr significant «expenditure» of Hsp70 was observed,
as well as the pronounced induction of «Hsp40»; the induction was expressed especially strongly in
24 hr after the stress application.
Key words: stress proteins, heat shock proteins, Porphyra, Rhodophyta
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