FATTY ACID PROFILE OF PSEUDOMONAS AURANTIACA DNA-BOUND LIPIDS ACCORDING
TO ESI-LC-MS MASS-SPECTROMETRY
R.I. Zhdanov,1,* D. Kern,2 W. Lorenz,2 M.Ya. Ibragimova 1
1 Chair of Biochemistry, Kazan (Volga Region) Federal University,
and 2 Institute of Analytical and Environmental Chemistry,
Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany;
* e-mail: zrenad@gmail.com
Approach to the study of prokaryotic chromatin lipidome has been realized based on analysis of fatty acid profile of DNA-bound lipids using electrospray ionization mass s
pectrometry ESI-LC-MS. By this method, we found 16 : 0 and 18 : 1 fatty acids, which are contained in the first fraction (weakly bound to DNA), and 14 : 0, 16 : 1 and 18 : 2
fatty acids, which are contained in the second fraction (strongly bound to DNA).
Key words: Pseudomonas aurantiaca, DNA-bound lipids, fatty acids, mass spectrometry
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