EXTRACELLULAR PROTEASOMES PURIFICATION METHODS AND THE EVALUATION OF PROTEASOMAL PEPTIDASE ACTIVITIES
Yu.Ya. Zaykova,1 V.A. Kulichkova,1 K.A. Mitenkova,2 A.S. Tsimokha 1,*
1 Institute of Cytology RAS, St. Petersburg, and 2 St. Petersburg State Polytechnical University;
* e-mail: atsimokha@mail.cytspb.rssi.ru
In this paper, we present a comparative analysis of different methods of purification of proteasomes from
the culture medium in which proerithroleukemia human K562 cells were grown. The results obtained allowed
us to purify proteasomes from samples of conditioned cell culture medium and control the quality of the proteasome
preparations at all stages of their separation. Extracellular proteasomes purified via different approaches
possess all the three types of peptidase activity described for intracellular counterparts.
Key words: extracellular proteasomes, peptidase activities, regulated proteolysis, ubiquitin-proteasome system
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